Pachlopnik Lab.

Inherited diseases that affect the immune system typically change the properties of immune cells. Most immune cells are present in the blood during their life cycle, allowing for sampling of a large range of immune cells with a minimally invasive technique.

Phenotypic profiling of a large number of single cells from each patient, using an unbiased comprehensive multivariate approach, enables the quantification of the phenotypic state, the molecular make-up, and the microenvironment of each individual cell. In conjunction with large-scale molecular perturbations this allows the generation of a highly quantitative multidimensional dataset from thousands of individual cells in thousands of different conditions per patient. 

 

By imaging patient's cells we hope to be able to better characterize and possibly predict the cellular pathways affected by their mutation. The ability to expose these cells to libraries of pharmaceutical compounds additionally enables screening for potential treatments.